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Clinically, in horses, used methods of assessing intestinal permeability in vivo include urine or serum recovery of enterally delivered radio-labeled markers including 51 Cr-ethylene diaminetetra-acetate ( 51 Cr-EDTA) [23], 99m Tc-diethylene triaminopenta-acetate ( 99m Tc-DPTA) [24], and poorly absorbed sugars such as D-xylose [25,26] and sucrose [27]

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For transwell experiments, inserts were placed in 50 mL centrifuge tubes containing 10 mL 1 × PBS (pH 7.4), and vortexed to separate the fungal cells from the membrane