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Cells were then plated at a density of 500 cells/cm 2 in mammosphere medium (DMEM-F12/B27/20-ng/ml EGF/PenStrep) in nonadherent conditions, in culture dishes coated with (2-hydroxyethylmethacrylate) (poly-HEMA, Sigma), in the presence of treatments, were required

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This experimental study was approved by the Investigational Review Board at Soonchunhyang University Cheonan Hospital (Cheonan, Korea), and all human subjects provided written informed consent, and their participation was voluntary