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Cell scratch experiment L929 cells were seeded in 6-well plates (8 × 10 5 cells per well) and cultured for 24 h to form a cell monolayer

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However, when excessive amounts of acetaminophen are ingested, the liver’s supply of glutathione can be depleted rapidly, leaving NAPQI unneutralized.[ref] Glutathione is the master antioxidant, but there are several situations in which glutathione levels may already be low and thus unable to rise to the occasion for high doses of acetaminophen